Hotspot

rps15-ycf1

SSC · 122536-122854 · 319 bp

Back to Erythranthe

Pi 0.0363

MarkerSeek score 62.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 319
variable_sites: 43
pi: 0.0363
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	AGCAGCCAAGTTTCACATGA	50.0	45.0	58.0	58.0	746-782 bp	1.0	89.4
rps15-ycf1_p2	AGTCGTTGACGTTTTCCCAA	AGCAGCCAAGTTTCACATGA	45.0	45.0	58.0	58.0	742-778 bp	1.0	89.3
rps15-ycf1_p3	CAGCAGTCGTTGACGTTTTC	GCAGCCAAGTTTCACATGAC	50.0	50.0	58.0	57.9	745-781 bp	1.0	89.1
rps15-ycf1_p4	CAGCAGTCGTTGACGTTTTC	CAGCAGCCAAGTTTCACATG	50.0	50.0	58.0	57.9	747-783 bp	1.0	89.1
rps15-ycf1_p5	TCCCAACGCGTTACATAAGG	AGCAGCCAAGTTTCACATGA	50.0	45.0	58.0	58.0	1136-1172 bp	1.0	88.3