Hotspot

ycf3-trnS-GGA

LSC · 45072-45733 · 662 bp

Back to Erythranthe

Pi 0.0244

MarkerSeek score 60.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 63
pi: 0.0244
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCAACGCCTCCAAATTTCCA	AACCATTTGACCCTTCACCC	45.0	50.0	58.0	58.0	1722-1746 bp	0.882353	79.0
ycf3-trnS-GGA_p2	TCAACGCCTCCAAATTTCCA	CTGGTGTAGGTCTCCATTGC	45.0	55.0	58.0	58.0	1377-1401 bp	0.882353	78.8
ycf3-trnS-GGA_p3	CAACCCTCCTGCAAGAGATC	AACCATTTGACCCTTCACCC	55.0	50.0	58.0	58.0	1805-1829 bp	0.882353	78.8
ycf3-trnS-GGA_p4	AACCCTCCTGCAAGAGATCT	AACCATTTGACCCTTCACCC	50.0	50.0	58.0	58.0	1804-1828 bp	0.823529	76.3
ycf3-trnS-GGA_p5	ACCCTCCTGCAAGAGATCTT	AACCATTTGACCCTTCACCC	50.0	50.0	58.0	58.0	1803-1827 bp	0.823529	76.3