Hotspot

psaA-ycf3

LSC · 42312-43116 · 805 bp

Back to Erythranthe

Pi 0.0247

MarkerSeek score 60.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 73
pi: 0.0247
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGTTTCGAATAA	50.0	45.0	57.9	57.6	668-916 bp	1.0	86.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCACGAGGCGTTTCGAATA	50.0	45.0	57.9	57.4	669-917 bp	1.0	85.1
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	TCACGAGGCGTTTCGAATAA	50.0	45.0	58.5	57.6	621-869 bp	1.0	84.3
psaA-ycf3_p4	TTTGGCCCATTCCTCGAAAG	TCACGAGGCGTTTCGAATAA	50.0	45.0	58.5	57.6	672-920 bp	1.0	84.1
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	GATCACGAGGCGTTTCGAAT	50.0	50.0	57.9	58.7	670-918 bp	1.0	83.9