Hotspot

trnG-GCC-trnfM-CAU

LSC · 37008-37179 · 172 bp

Back to Erythranthe

Pi 0.0443
MarkerSeek score 63.80
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
172
variable_sites
31
pi
0.0443
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 273-277 bp 1.0 87.9
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 274-278 bp 1.0 87.9
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 237-241 bp 1.0 87.9
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 238-242 bp 1.0 87.9
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC GTCACGGGTTCAAATCCTGT 50.0 50.0 58.1 58.1 231-235 bp 1.0 87.4