Hotspot

petN-psbM

LSC · 29712-30716 · 1005 bp

Back to Erythranthe

Pi 0.0223

MarkerSeek score 61.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 82
pi: 0.0223
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTCCCGACGGATCCAATAT	50.0	50.0	58.0	58.0	1660-1703 bp	1.0	89.6
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	AGTCCCGACGGATCCAATAT	50.0	50.0	58.0	58.0	1658-1701 bp	1.0	89.6
petN-psbM_p3	TCGTAGTGTGGGGAAGAAGT	GAAGTGGAAAGGCAACCTGA	50.0	50.0	58.0	58.0	1464-1499 bp	1.0	88.1
petN-psbM_p4	CGAAAATCTCGGAGTGCTCA	AGTCCCGACGGATCCAATAT	50.0	50.0	58.0	58.0	2236-2271 bp	0.705882	70.9
petN-psbM_p5	CGAAAATCTCGGAGTGCTCA	GAAGTGGAAAGGCAACCTGA	50.0	50.0	58.0	58.0	2050-2071 bp	0.705882	69.4