Hotspot

rpoB-trnC-GCA

LSC · 27500-28704 · 1205 bp

Back to Erythranthe

Pi 0.0222
MarkerSeek score 59.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1205
variable_sites
97
pi
0.0222
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GAAAATACCGGATCCCCACC CGCCTGTTTCCTATGCTTCT 55.0 50.0 58.0 58.0 2054-2101 bp 0.882353 78.0
rpoB-trnC-GCA_p2 CGAGCCCATATCCTTGCTTT ATACCTAGTAACGTCCCGGG 50.0 55.0 58.0 58.0 2427-2477 bp 0.823529 77.1
rpoB-trnC-GCA_p3 GAAAATACCGGATCCCCACC ATACCTAGTAACGTCCCGGG 55.0 55.0 58.0 58.0 2634-2684 bp 0.823529 76.8
rpoB-trnC-GCA_p4 CGAGCCCATATCCTTGCTTT TCGCCTGTTTCCTATGCTTC 50.0 50.0 58.0 58.0 1848-1895 bp 0.705882 69.6
rpoB-trnC-GCA_p5 GAAAATACCGGATCCCCACC TCGCCTGTTTCCTATGCTTC 55.0 50.0 58.0 58.0 2055-2102 bp 0.705882 69.4