Hotspot

psbI-trnS-GCU

LSC · 8706-8826 · 121 bp

Back to Erythranthe

Pi 0.0574

MarkerSeek score 61.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 28
pi: 0.0574
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	718-730 bp	1.0	87.6
psbI-trnS-GCU_p2	CTTTTGTTTGGCAAGCTGCT	TGGACTAAAGCGTCGGATTG	45.0	50.0	58.1	58.0	723-735 bp	1.0	87.5
psbI-trnS-GCU_p3	CTTTTGTTTGGCAAGCTGCT	GTGGACTAAAGCGTCGGATT	45.0	50.0	58.1	58.0	724-736 bp	1.0	87.5
psbI-trnS-GCU_p4	TTCTTGGCTTGGATAGTCGC	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	586-588 bp	0.705882	68.6
psbI-trnS-GCU_p5	TTCTTGGCTTGGATAGTCGC	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.0	58.0	587-589 bp	0.705882	68.5