Hotspot

trnH-GUG-psbA

LSC · 93-502 · 410 bp

Back to Erythranthe

Pi 0.0483

MarkerSeek score 74.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 75
pi: 0.0483
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	467-607 bp	1.0	90.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	481-621 bp	1.0	90.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	480-620 bp	1.0	90.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	482-622 bp	1.0	90.3
trnH-GUG-psbA_p5	GCCTTGATCCACTTGGCTAC	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.6	57.9	472-612 bp	1.0	86.5