Hotspot

ycf1

SSC · 123508-128883 · 5376 bp

Back to Erysimum

Pi 0.00244

MarkerSeek score 48.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5376
variable_sites: 54
pi: 0.00244
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTCTTGTTGTGGTTCTGA	TGATCCATTTTTGCATGGGA	40.0	40.0	54.4	55.6	234 bp	1.0	42.4
ycf1_p2	TCTTCTTGTTGTGGTTCTGA	TCCATTTTTGCATGGGATCTC	40.0	42.9	54.4	56.5	231 bp	1.0	42.4
ycf1_p3	TCTTCTTGTTGTGGTTCTGAA	TGATCCATTTTTGCATGGGA	38.1	40.0	55.3	55.6	234 bp	1.0	42.4
ycf1_p4	TTCTTCTTGTTGTGGTTCTGA	TGATCCATTTTTGCATGGGA	38.1	40.0	55.3	55.6	235 bp	1.0	42.4
ycf1_p5	TCTTCTTGTTGTGGTTCTGAA	TCCATTTTTGCATGGGATCTC	38.1	42.9	55.3	56.5	231 bp	1.0	42.4