Hotspot

ndhA

SSC · 119481-121626 · 2146 bp

Back to Erysimum

Pi 0.00183

MarkerSeek score 44.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2146
variable_sites: 18
pi: 0.00183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTATCATAGGAAGCA	TGACGATACTCGGTAGCATAGA	42.9	45.5	56.1	58.0	2303-2325 bp	1.0	41.6
ndhA_p2	ACCCAGTTATCATAGGAAGCA	ATGACGATACTCGGTAGCAT	42.9	45.0	56.1	55.9	2304-2326 bp	1.0	41.2
ndhA_p3	GAACCCAGTTATCATAGGAAGCA	TGACGATACTCGGTAGCATAGA	43.5	45.5	58.0	58.0	2305-2327 bp	1.0	40.7
ndhA_p4	TGAACCCAGTTATCATAGGAAGC	TGACGATACTCGGTAGCATAGA	43.5	45.5	58.0	58.0	2306-2328 bp	1.0	40.7
ndhA_p5	GAACCCAGTTATCATAGGAAGCA	ATGACGATACTCGGTAGCAT	43.5	45.0	58.0	55.9	2306-2328 bp	1.0	40.7