Hotspot

ndhF-rpl32

SSC · 112237-113062 · 826 bp

Back to Erysimum

Pi 0.00444

MarkerSeek score 47.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 826
variable_sites: 15
pi: 0.00444
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGATGATCCATGAATATTGA	CGATGTCCAATATCCCTTCCT	38.1	47.6	52.8	56.9	899-955 bp	1.0	45.3
ndhF-rpl32_p2	GGGATGATCCATGAATATTGA	ACGATGTCCAATATCCCTTCC	38.1	47.6	52.8	57.2	900-956 bp	1.0	45.2
ndhF-rpl32_p3	GGGATGATCCATGAATATTGA	AACGATGTCCAATATCCCTTCC	38.1	45.5	52.8	57.9	901-957 bp	1.0	45.2
ndhF-rpl32_p4	GGGATGATCCATGAATATTGAT	ACGATGTCCAATATCCCTTCC	36.4	47.6	53.5	57.2	900-956 bp	1.0	45.2
ndhF-rpl32_p5	GGGATGATCCATGAATATTGA	AAGCTTTCAACGATGTCCAA	38.1	40.0	52.8	55.9	909-965 bp	1.0	45.2