Hotspot

psbE-petL

LSC · 63712-64950 · 1239 bp

Back to Erysimum

Pi 0.00246

MarkerSeek score 54.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1239
variable_sites: 13
pi: 0.00246
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACCAGCCTGCAATGAATAGG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1381-1387 bp	1.0	67.0
psbE-petL_p2	ACGAATAACCAGCCTGCAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	1388-1394 bp	1.0	66.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1302-1308 bp	1.0	66.3
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1297-1303 bp	1.0	66.0
psbE-petL_p5	ACCAGCCTGCAATGAATAGG	AGCTGCTAGTAGAAAACCGAA	50.0	42.9	57.9	57.0	1381-1387 bp	1.0	65.1