Hotspot

psaI-ycf4

LSC · 58693-59090 · 398 bp

Back to Erysimum

Pi 0.00212

MarkerSeek score 51.50

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 398
variable_sites: 4
pi: 0.00212
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GGCAATTGCAATGGCTTCTT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.9	58.0	481-515 bp	1.0	83.5
psaI-ycf4_p2	TAGTCTTTCCGGCAATTGCA	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.8	58.0	491-525 bp	1.0	82.7
psaI-ycf4_p3	GGCAATTGCAATGGCTTCTT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	505-539 bp	1.0	82.6
psaI-ycf4_p4	AGTAGGCCTAGTCTTTCCGG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.2	58.0	499-533 bp	1.0	82.5
psaI-ycf4_p5	GCCTAGTCTTTCCGGCAATT	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.2	58.0	494-528 bp	1.0	82.3