Hotspot

trnF-UUC-ndhJ

LSC · 47906-48516 · 611 bp

Back to Erysimum

Pi 0.00189

MarkerSeek score 41.00

Primer available yes

Label trnF-UUC-ndhJ

Feature Metadata

label_name: trnF-UUC-ndhJ
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 5
pi: 0.00189
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-UUC-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	750-768 bp	1.0	80.1
trnF-UUC-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCTAATGCCCGAAAGTTGGA	50.0	50.0	58.1	57.9	730-748 bp	1.0	79.3
trnF-UUC-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TCCTAATGCCCGAAAGTTGG	50.0	50.0	58.1	57.9	731-749 bp	1.0	79.3
trnF-UUC-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGATAGCCATCCACGACTGA	50.0	50.0	58.1	58.2	757-775 bp	1.0	78.4
trnF-UUC-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCCACGACTGAAACGGATC	50.0	50.0	58.1	57.7	749-767 bp	1.0	77.9