Hotspot

trnL-UUA-trnF-UUC

LSC · 47432-47832 · 401 bp

Back to Erysimum

Pi 0.0173

MarkerSeek score 63.30

Primer available yes

Label trnL-UUA-trnF-UUC

Feature Metadata

label_name: trnL-UUA-trnF-UUC
feature_type: igs
region: LSC
length_bp: 401
variable_sites: 25
pi: 0.0173
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUA-trnF-UUC_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	491-871 bp	1.0	80.3
trnL-UUA-trnF-UUC_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	488-868 bp	1.0	77.1
trnL-UUA-trnF-UUC_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	480-860 bp	1.0	76.5
trnL-UUA-trnF-UUC_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	453-833 bp	1.0	76.3
trnL-UUA-trnF-UUC_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	463-843 bp	1.0	76.1