Hotspot

trnT-ACA-trnL-UUA

LSC · 46146-46841 · 696 bp

Back to Erysimum

Pi 0.00405
MarkerSeek score 55.10
Primer available yes
Label trnT-ACA-trnL-UUA

Feature Metadata

label_name
trnT-ACA-trnL-UUA
feature_type
igs
region
LSC
length_bp
696
variable_sites
11
pi
0.00405
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACA-trnL-UUA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.9 58.2 757-772 bp 1.0 80.0
trnT-ACA-trnL-UUA_p2 TATGGAAAAGCCGGCTATCG CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 808-823 bp 1.0 79.6
trnT-ACA-trnL-UUA_p3 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 760-775 bp 1.0 79.3
trnT-ACA-trnL-UUA_p4 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 789-804 bp 1.0 79.1
trnT-ACA-trnL-UUA_p5 TATGGAAAAGCCGGCTATCG AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 811-826 bp 1.0 78.9