Hotspot

psaA-ycf3

LSC · 41711-42418 · 708 bp

Back to Erysimum

Pi 0.003

MarkerSeek score 56.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 7
pi: 0.003
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	768-821 bp	1.0	83.8
psaA-ycf3_p2	AGAAATGACCGGGTTTAGCC	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	785-838 bp	1.0	83.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCACAGAATTGGTTGACGA	50.0	45.0	57.9	57.7	776-829 bp	1.0	81.8
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCCGCTCAGTATTGGAAAC	50.0	50.0	57.9	58.3	830-883 bp	1.0	81.7
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTCAGTATTGGAA	50.0	50.0	57.9	58.3	832-885 bp	1.0	81.7