Hotspot

trnR-AGA-atpA

LSC · 9527-9815 · 289 bp

Back to Erysimum

Pi 0.00715

MarkerSeek score 46.30

Primer available yes

Label trnR-AGA-atpA

Feature Metadata

label_name: trnR-AGA-atpA
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 7
pi: 0.00715
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-AGA-atpA_p1	GCGAAAAGCGTCCATTGTC	ACATTAACCGCTGAAGCAGA	52.6	45.0	58.0	57.5	443-452 bp	1.0	71.0
trnR-AGA-atpA_p2	GCGAAAAGCGTCCATTGTC	AGACATTAACCGCTGAAGCA	52.6	45.0	58.0	57.5	445-454 bp	1.0	71.0
trnR-AGA-atpA_p3	GCGAAAAGCGTCCATTGTC	GACATTAACCGCTGAAGCAG	52.6	50.0	58.0	57.2	444-453 bp	1.0	68.3
trnR-AGA-atpA_p4	GCGAAAAGCGTCCATTGTC	TAACCGCTGAAGCAGAAAGC	52.6	50.0	58.0	58.8	439-448 bp	1.0	68.1
trnR-AGA-atpA_p5	GCGAAAAGCGTCCATTGTC	ACCAAGACATTAACCGCTGA	52.6	45.0	58.0	57.1	449-458 bp	1.0	68.0