Hotspot

trnH-CAC-psbA

LSC · 77-373 · 297 bp

Back to Erysimum

Pi 0.0139

MarkerSeek score 58.70

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 297
variable_sites: 14
pi: 0.0139
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	333-372 bp	1.0	85.7
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	GAGGCTCCATCTACAAACGG	50.0	55.0	57.9	58.1	327-366 bp	1.0	85.7
trnH-CAC-psbA_p3	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	348-387 bp	1.0	85.5
trnH-CAC-psbA_p4	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	334-373 bp	1.0	85.5
trnH-CAC-psbA_p5	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	351-390 bp	1.0	85.4