Hotspot

rpl32-trnL-UAG

SSC · 115886-116844 · 959 bp

Back to Eryngium

Pi 0.0461

MarkerSeek score 79.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 959
variable_sites: 63
pi: 0.0461
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGGATATTGGGCAGCCTTA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.2	58.1	1092-1110 bp	1.0	86.8
rpl32-trnL-UAG_p2	AGGGATATTGGGCAGCCTTA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.2	58.1	1094-1112 bp	1.0	86.8
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1081-1099 bp	1.0	86.5
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCCTTA	GCCTTGGGTAAAAATGACGC	50.0	50.0	58.2	58.0	1536-1551 bp	0.666667	66.8
rpl32-trnL-UAG_p5	GCAGCCTTAAAAGCGTTGTC	GCCTTGGGTAAAAATGACGC	50.0	50.0	58.3	58.0	1525-1540 bp	0.666667	65.7