Hotspot

accD-psaI

LSC · 60947-61279 · 333 bp

Back to Eryngium

Pi 0.071

MarkerSeek score 78.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 333
variable_sites: 32
pi: 0.071
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	CCAAAACAGAGGGAAAGTCGA	50.0	47.6	58.8	58.2	348-407 bp	1.0	75.4
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	CCAAAACAGAGGGAAAGTCG	50.0	50.0	58.8	56.6	348-407 bp	1.0	73.8
accD-psaI_p3	GCTCCACGCTTTCTTTCCTT	ACCAAAACAGAGGGAAAGTCG	50.0	47.6	58.8	58.4	349-408 bp	1.0	73.2
accD-psaI_p4	GCTCCACGCTTTCTTTCCT	CCAAAACAGAGGGAAAGTCGA	52.6	47.6	58.1	58.2	348-407 bp	1.0	73.0
accD-psaI_p5	TCCACGCTTTCTTTCCTTTGA	CCAAAACAGAGGGAAAGTCGA	42.9	47.6	58.1	58.2	346-405 bp	1.0	72.9