Hotspot

psbC-trnS-UGA

LSC · 36733-36991 · 259 bp

Back to Eryngium

Pi 0.0618

MarkerSeek score 80.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 20
pi: 0.0618
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	356-393 bp	1.0	86.8
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	395-432 bp	1.0	86.6
psbC-trnS-UGA_p3	GCAGCAGGGTTTGAAAAAGG	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.1	58.1	387-424 bp	1.0	86.5
psbC-trnS-UGA_p4	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	380-417 bp	1.0	86.5
psbC-trnS-UGA_p5	GCAGCAGGGTTTGAAAAAGG	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.1	58.1	388-425 bp	1.0	86.4