Hotspot

trnT-GGU-psbD

LSC · 33315-34301 · 987 bp

Back to Eryngium

Pi 0.0312

MarkerSeek score 64.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 987
variable_sites: 36
pi: 0.0312
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGACTCGCACTACGGAAATT	AGACCGGACCATCCTACAAA	50.0	50.0	58.0	58.1	1354-1601 bp	1.0	86.7
trnT-GGU-psbD_p2	GGACTCGCACTACGGAAATT	TCAGGGCCCCATAGTAACAA	50.0	50.0	58.0	58.0	1537-1784 bp	1.0	86.5
trnT-GGU-psbD_p3	GGACTCGCACTACGGAAATT	TTCAGGGCCCCATAGTAACA	50.0	50.0	58.0	58.0	1538-1785 bp	1.0	86.5
trnT-GGU-psbD_p4	GGACTCGCACTACGGAAATT	CAGGGCCCCATAGTAACAAC	50.0	55.0	58.0	57.9	1536-1783 bp	1.0	86.3
trnT-GGU-psbD_p5	ATTGCGAGGTGTGCATGTAT	TCAGGGCCCCATAGTAACAA	45.0	50.0	58.0	58.0	1393-1640 bp	1.0	85.7