Hotspot

trnC-GCA-petN

LSC · 29381-30220 · 840 bp

Back to Eryngium

Pi 0.0708

MarkerSeek score 87.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 80
pi: 0.0708
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	822-896 bp	1.0	89.6
trnC-GCA-petN_p2	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	55.0	50.0	57.9	58.2	862-936 bp	1.0	89.4
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	840-914 bp	1.0	87.2
trnC-GCA-petN_p4	GATATGGCCGAGTGGTAAGG	AGCCCAAGCGAGACTTACTA	55.0	50.0	57.5	58.2	861-935 bp	1.0	86.2
trnC-GCA-petN_p5	CGATATGGCCGAGTGGTAAG	GCCCAAGCGAGACTTACTAT	55.0	50.0	57.9	57.1	861-935 bp	1.0	83.4