Hotspot

rpoB-trnC-GCA

LSC · 28028-29309 · 1282 bp

Back to Eryngium

Pi 0.0367

MarkerSeek score 84.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1282
variable_sites: 70
pi: 0.0367
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCAAGCCCTGATCAATG	CTTACCACTCGGCCATATCG	55.0	55.0	58.3	57.9	1373-1401 bp	1.0	85.0
rpoB-trnC-GCA_p2	CCCTCAAGCCCTGATCAATG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.3	58.1	1413-1441 bp	1.0	84.8
rpoB-trnC-GCA_p3	TCCCTCAAGCCCTGATCAAT	CTTACCACTCGGCCATATCG	50.0	55.0	58.4	57.9	1374-1402 bp	1.0	84.3
rpoB-trnC-GCA_p4	TCCCTCAAGCCCTGATCAAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1414-1442 bp	1.0	84.1
rpoB-trnC-GCA_p5	CCCTCAAGCCCTGATCAATG	TTATTGATCAGGCGACACCC	55.0	50.0	58.3	57.7	1431-1459 bp	1.0	82.8