Hotspot

atpH-atpI

LSC · 14516-15678 · 1163 bp

Back to Eryngium

Pi 0.0264

MarkerSeek score 73.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 46
pi: 0.0264
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1250-1260 bp	1.0	79.5
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1306-1316 bp	1.0	78.7
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1266-1276 bp	1.0	78.6
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1251-1261 bp	1.0	78.3
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.2	58.5	1310-1320 bp	1.0	78.1