Hotspot

ycf1

Genome · 133719-139466 · 5748 bp

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Pi 0.334

MarkerSeek score 72.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5748
variable_sites: 1257
pi: 0.334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGAAGAACCAAAGTTGCA	CCTTGTTTGCGATAAGGGGA	45.0	50.0	57.8	57.9	389 bp	0.5	57.8
ycf1_p2	TGGGAAGAACCAAAGTTGCA	TCCTTGTTTGCGATAAGGGG	45.0	50.0	57.8	57.9	390 bp	0.5	57.8
ycf1_p3	TGGGAAGAACCAAAGTTGCA	AAAATATCGCAAACCGGGGT	45.0	45.0	57.8	58.2	906 bp	0.5	57.5
ycf1_p4	TGGGAAGAACCAAAGTTGCA	AAATATCGCAAACCGGGGTT	45.0	45.0	57.8	58.2	905 bp	0.5	57.5
ycf1_p5	TGGGAAGAACCAAAGTTGCA	CTTCAGCGGTCTAGAACGAG	45.0	55.0	57.8	57.8	762 bp	0.5	57.3