Hotspot

ndhI

Genome · 128722-129225 · 504 bp

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Pi 0.442

MarkerSeek score 74.00

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: Genome
length_bp: 504
variable_sites: 145
pi: 0.442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	ACCCCCTAATAGAAGACCCG	TGACGACTTGCCTCAAACAA	55.0	45.0	57.9	58.0	968 bp	0.5	59.0
ndhI_p2	ACCCCCTAATAGAAGACCCG	TTGACGACTTGCCTCAAACA	55.0	45.0	57.9	58.0	969 bp	0.5	59.0
ndhI_p3	CCATCTCCAAGGGTCCAAAG	TGACGACTTGCCTCAAACAA	55.0	45.0	57.9	58.0	1192 bp	0.5	58.7
ndhI_p4	CCATCTCCAAGGGTCCAAAG	TTGACGACTTGCCTCAAACA	55.0	45.0	57.9	58.0	1193 bp	0.5	58.7
ndhI_p5	CCCAAGGAAAAGGCCGAATA	TGACGACTTGCCTCAAACAA	50.0	45.0	57.9	58.0	1018 bp	0.5	58.6