Hotspot

clpP

Genome · 71766-72002 · 237 bp

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Pi 0.0717

MarkerSeek score 80.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 237
variable_sites: 17
pi: 0.0717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CACGTATTTGAGCGAAATCCA	CAATGCCCAAAAGATGCCTG	42.9	50.0	57.0	58.0	406 bp	1.0	67.1
clpP_p2	CACGTATTTGAGCGAAATCCA	AGATGCCTGTACGGTTGTTC	42.9	50.0	57.0	57.9	395 bp	1.0	66.6
clpP_p3	CACGTATTTGAGCGAAATCCA	CCAAAAGATGCCTGTACGGT	42.9	50.0	57.0	58.2	400 bp	1.0	65.9
clpP_p4	CACGTATTTGAGCGAAATCCA	GATGCCTGTACGGTTGTTCA	42.9	50.0	57.0	58.2	394 bp	1.0	65.8
clpP_p5	ACAATCCCTACAAGATCAACCC	CAATGCCCAAAAGATGCCTG	45.5	50.0	57.8	58.0	378 bp	1.0	65.6