Hotspot

5S ribosomal RNA-4.5S ribosomal RNA

Genome · 141113-141325 · 213 bp

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Pi 0.0704
MarkerSeek score 79.10
Primer available yes
Label 5S ribosomal RNA-4.5S ribosomal RNA

Feature Metadata

label_name
5S ribosomal RNA-4.5S ribosomal RNA
feature_type
igs
region
Genome
length_bp
213
variable_sites
15
pi
0.0704
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
5S ribosomal RNA-4.5S ribosomal RNA_p1 AGTATCGTCACCGCAGTAGA AGGCATCCTAACAGACCAGT 50.0 50.0 58.0 58.0 324-329 bp 1.0 83.1
5S ribosomal RNA-4.5S ribosomal RNA_p2 TATCGTCACCGCAGTAGAGT AGGCATCCTAACAGACCAGT 50.0 50.0 58.0 58.0 322-327 bp 1.0 83.1
5S ribosomal RNA-4.5S ribosomal RNA_p3 TATCGTCACCGCAGTAGAGT TGAGGCATCCTAACAGACCA 50.0 50.0 58.0 58.0 324-329 bp 1.0 83.0
5S ribosomal RNA-4.5S ribosomal RNA_p4 AGTATCGTCACCGCAGTAGA GTCAAGTGGAAGTGCAGTGA 50.0 50.0 58.0 58.0 356-361 bp 1.0 82.7
5S ribosomal RNA-4.5S ribosomal RNA_p5 TATCGTCACCGCAGTAGAGT GTCAAGTGGAAGTGCAGTGA 50.0 50.0 58.0 58.0 354-359 bp 1.0 82.7