Hotspot

rps15-ycf1

Genome · 133361-133718 · 358 bp

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Pi 0.451

MarkerSeek score 69.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: Genome
length_bp: 358
variable_sites: 96
pi: 0.451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.59
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GCTCAATCGTTCAGGTCCAT	AACCCCTTCGACTGTCTGTA	50.0	50.0	58.0	58.0	1493 bp	0.5	59.7
rps15-ycf1_p2	GCTCAATCGTTCAGGTCCAT	ACCCCTTCGACTGTCTGTAA	50.0	50.0	58.0	58.0	1492 bp	0.5	59.7
rps15-ycf1_p3	GCTCAATCGTTCAGGTCCAT	ATGGCGTGGATACCAATTCC	50.0	50.0	58.0	58.0	1268 bp	0.5	59.6
rps15-ycf1_p4	GCTCAATCGTTCAGGTCCAT	ATTGGAATGGCGTGGATACC	50.0	50.0	58.0	58.0	1274 bp	0.5	59.6
rps15-ycf1_p5	GCTCAATCGTTCAGGTCCAT	GGAATGGCGTGGATACCAAT	50.0	50.0	58.0	58.0	1271 bp	0.5	59.6