primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	clpP	1	CACGTATTTGAGCGAAATCCA	CAATGCCCAAAAGATGCCTG	21	20	42.9	50.0	57.0	58.0	0.0	0.0	2.027351	71766	72002	406-406	1.000000	67.1
clpP_p2	clpP	2	CACGTATTTGAGCGAAATCCA	AGATGCCTGTACGGTTGTTC	21	20	42.9	50.0	57.0	57.9	0.0	0.0	2.103572	71766	72002	395-395	1.000000	66.6
clpP_p3	clpP	3	CACGTATTTGAGCGAAATCCA	CCAAAAGATGCCTGTACGGT	21	20	42.9	50.0	57.0	58.2	0.0	0.0	2.187593	71766	72002	400-400	1.000000	65.9
clpP_p4	clpP	4	CACGTATTTGAGCGAAATCCA	GATGCCTGTACGGTTGTTCA	21	20	42.9	50.0	57.0	58.2	0.0	0.0	2.205475	71766	72002	394-394	1.000000	65.8
clpP_p5	clpP	5	ACAATCCCTACAAGATCAACCC	CAATGCCCAAAAGATGCCTG	22	20	45.5	50.0	57.8	58.0	0.0	0.0	2.255069	71766	72002	378-378	1.000000	65.6
4.5S ribosomal RNA-5S ribosomal RNA_p1	4.5S ribosomal RNA-5S ribosomal RNA	1	AGGCATCCTAACAGACCAGT	AGTATCGTCACCGCAGTAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.075816	108771	108983	324-329	1.000000	83.1
4.5S ribosomal RNA-5S ribosomal RNA_p2	4.5S ribosomal RNA-5S ribosomal RNA	2	AGGCATCCTAACAGACCAGT	TATCGTCACCGCAGTAGAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.075816	108771	108983	322-327	1.000000	83.1
4.5S ribosomal RNA-5S ribosomal RNA_p3	4.5S ribosomal RNA-5S ribosomal RNA	3	TGAGGCATCCTAACAGACCA	TATCGTCACCGCAGTAGAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.082895	108771	108983	324-329	1.000000	83.0
4.5S ribosomal RNA-5S ribosomal RNA_p4	4.5S ribosomal RNA-5S ribosomal RNA	4	GTCAAGTGGAAGTGCAGTGA	AGTATCGTCACCGCAGTAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	108771	108983	356-361	1.000000	82.7
4.5S ribosomal RNA-5S ribosomal RNA_p5	4.5S ribosomal RNA-5S ribosomal RNA	5	GTCAAGTGGAAGTGCAGTGA	TATCGTCACCGCAGTAGAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	108771	108983	354-359	1.000000	82.7
ndhF-rpl32_p1	ndhF-rpl32	1	GCACCGACAAATAATAGCGC	TAAGAGCGAGGCGAGTTCTA	20	20	50.0	50.0	58.0	58.0	0.0	2.7	0.052821	121888	122611	1720-1720	0.500000	59.6
ndhF-rpl32_p2	ndhF-rpl32	2	GCACCGACAAATAATAGCGC	GAAGAGATAAGAGCGAGGCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.063605	121888	122611	1727-1727	0.500000	59.5
ndhF-rpl32_p3	ndhF-rpl32	3	ATCCTAACATGGCAGCACTG	TAAGAGCGAGGCGAGTTCTA	20	20	50.0	50.0	58.0	58.0	0.0	2.7	0.082548	121888	122611	1422-1422	0.500000	59.3
ndhF-rpl32_p4	ndhF-rpl32	4	ATCCTAACATGGCAGCACTG	GAAGAGATAAGAGCGAGGCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.093332	121888	122611	1429-1429	0.500000	59.3
ndhF-rpl32_p5	ndhF-rpl32	5	AGAAGTCAGTGGGTCGATCA	TAAGAGCGAGGCGAGTTCTA	20	20	50.0	50.0	58.1	58.0	0.0	2.7	0.109373	121888	122611	1292-1292	0.500000	59.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGAAGAGCTTTTGGACAGC	CCCTGGGTAAAAATAGCGCT	20	20	50.0	50.0	58.1	57.9	0.0	17.3	0.149844	122786	123803	1669-1669	0.500000	58.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGAAAATCCCTTTCGACCG	CCCTGGGTAAAAATAGCGCT	20	20	50.0	50.0	57.9	57.9	0.0	17.3	0.162207	122786	123803	1631-1631	0.500000	58.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGAAGAGCTTTTGGACAGC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.221339	122786	123803	1168-1168	0.500000	58.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGAAGAGCTTTTGGACAGC	GAGAAGAGTCTAGCCCCCAA	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.229699	122786	123803	1316-1316	0.500000	58.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGAAAATCCCTTTCGACCG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.233702	122786	123803	1130-1130	0.500000	58.1
ccsA_p1	ccsA	1	TAGACACGCTGCTCTTAGGA	TGCTGCAACCTCTTGGATTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124629	124012	124953	1170-1170	0.500000	59.0
ccsA_p2	ccsA	2	TTGGGGGCTAGACTCTTCTC	TGCTGCAACCTCTTGGATTT	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.132989	124012	124953	1022-1022	0.500000	58.9
ccsA_p3	ccsA	3	TAGACACGCTGCTCTTAGGA	GGTATGAGCGGTTTTGTTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.147089	124012	124953	1721-1721	0.500000	58.8
ccsA_p4	ccsA	4	TAGACACGCTGCTCTTAGGA	GTATGAGCGGTTTTGTTGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.147089	124012	124953	1720-1720	0.500000	58.8
ccsA_p5	ccsA	5	TTGGGGGCTAGACTCTTCTC	GTATGAGCGGTTTTGTTGCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.155449	124012	124953	1572-1572	0.500000	58.8
ndhD_p1	ndhD	1	AAATCCAAGAGGTTGCAGCA	GCTATTGGGCTGGCTATTGT	20	20	45.0	50.0	58.0	58.0	2.0	0.0	0.013469	125199	126737	2442-2442	0.500000	59.9
ndhD_p2	ndhD	2	AAATCCAAGAGGTTGCAGCA	CAAATTGCTTCCGCTCCAAG	20	20	45.0	50.0	58.0	58.0	2.0	0.0	0.013654	125199	126737	1988-1988	0.500000	59.9
ndhD_p3	ndhD	3	AAATCCAAGAGGTTGCAGCA	AGATGTGAATCCGCTTGTCC	20	20	45.0	50.0	58.0	58.0	2.0	0.0	0.030190	125199	126737	1943-1943	0.500000	59.8
ndhD_p4	ndhD	4	AAATCCAAGAGGTTGCAGCA	AAATTGCTTCCGCTCCAAGA	20	20	45.0	45.0	58.0	58.1	2.0	0.0	0.090652	125199	126737	1987-1987	0.500000	59.3
ndhD_p5	ndhD	5	AAATCCAAGAGGTTGCAGCA	AATTGCTTCCGCTCCAAGAA	20	20	45.0	45.0	58.0	58.1	2.0	0.0	0.090652	125199	126737	1986-1986	0.500000	59.3
ndhD-psaC_p1	ndhD-psaC	1	AGGGCCTACAGAAAGTCCAT	GCTATTGGGCTGGCTATTGT	20	20	50.0	50.0	58.0	58.0	2.8	0.0	0.040530	126738	126825	971-971	0.500000	59.7
ndhD-psaC_p2	ndhD-psaC	2	AGGGCCTACAGAAAGTCCAT	CAAATTGCTTCCGCTCCAAG	20	20	50.0	50.0	58.0	58.0	2.8	0.0	0.040714	126738	126825	517-517	0.500000	59.7
ndhD-psaC_p3	ndhD-psaC	3	GGTCCCGAGAAGCAAATGAT	GCTATTGGGCTGGCTATTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057240	126738	126825	1104-1104	0.500000	59.5
ndhD-psaC_p4	ndhD-psaC	4	AGGGCCTACAGAAAGTCCAT	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	58.0	58.0	2.8	0.0	0.057250	126738	126825	472-472	0.500000	59.5
ndhD-psaC_p5	ndhD-psaC	5	GGTCCCGAGAAGCAAATGAT	CAAATTGCTTCCGCTCCAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057425	126738	126825	650-650	0.500000	59.5
psaC_p1	psaC	1	AGGGCCTACAGAAAGTCCAT	GCTATTGGGCTGGCTATTGT	20	20	50.0	50.0	58.0	58.0	2.8	0.0	0.040530	126826	127071	971-971	0.500000	59.7
psaC_p2	psaC	2	GGTCCCGAGAAGCAAATGAT	GCTATTGGGCTGGCTATTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057240	126826	127071	1104-1104	0.500000	59.5
psaC_p3	psaC	3	AGACTTGGACCACAAAACCC	GCTATTGGGCTGGCTATTGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.080483	126826	127071	690-690	0.500000	59.4
psaC_p4	psaC	4	AGGGCCTACAGAAAGTCCAT	GGCTGGCTATTGTTTCCTCA	20	20	50.0	50.0	58.0	57.9	2.8	0.0	0.156183	126826	127071	964-964	0.500000	58.8
psaC_p5	psaC	5	GGTCCCGAGAAGCAAATGAT	GGCTGGCTATTGTTTCCTCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.172893	126826	127071	1097-1097	0.500000	58.6
ndhG_p1	ndhG	1	ACAATAGCCAGCCCAATAGC	TACCCGTTGTTGATTGGCAA	20	20	50.0	45.0	58.0	58.0	0.0	0.7	0.030457	127858	128388	1599-1599	0.500000	59.8
ndhG_p2	ndhG	2	ACAATAGCCAGCCCAATAGC	TTACCCGTTGTTGATTGGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.030457	127858	128388	1600-1600	0.500000	59.8
ndhG_p3	ndhG	3	TGAGGAAACAATAGCCAGCC	TACCCGTTGTTGATTGGCAA	20	20	50.0	45.0	57.9	58.0	0.0	0.7	0.146110	127858	128388	1606-1606	0.500000	58.8
ndhG_p4	ndhG	4	TGAGGAAACAATAGCCAGCC	TTACCCGTTGTTGATTGGCA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.146110	127858	128388	1607-1607	0.500000	58.8
ndhG_p5	ndhG	5	CAATAGCCAGCCCAATAGCT	TACCCGTTGTTGATTGGCAA	20	20	50.0	45.0	57.7	58.0	0.0	0.7	0.300485	127858	128388	1598-1598	0.500000	57.6
ndhI_p1	ndhI	1	ACCCCCTAATAGAAGACCCG	TGACGACTTGCCTCAAACAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.129876	128722	129225	968-968	0.500000	59.0
ndhI_p2	ndhI	2	ACCCCCTAATAGAAGACCCG	TTGACGACTTGCCTCAAACA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.129876	128722	129225	969-969	0.500000	59.0
ndhI_p3	ndhI	3	CCATCTCCAAGGGTCCAAAG	TGACGACTTGCCTCAAACAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.166047	128722	129225	1192-1192	0.500000	58.7
ndhI_p4	ndhI	4	CCATCTCCAAGGGTCCAAAG	TTGACGACTTGCCTCAAACA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.166047	128722	129225	1193-1193	0.500000	58.7
ndhI_p5	ndhI	5	CCCAAGGAAAAGGCCGAATA	TGACGACTTGCCTCAAACAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.169068	128722	129225	1018-1018	0.500000	58.6
ndhA_p1	ndhA	1	TGCCAATCAACAACGGGTAA	TACTCCCCACGATCCTGATC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.020541	129308	129860	1492-1492	0.500000	59.8
ndhA_p2	ndhA	2	TTGCCAATCAACAACGGGTA	TACTCCCCACGATCCTGATC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.020541	129308	129860	1493-1493	0.500000	59.8
ndhA_p3	ndhA	3	TGTTTGAGGCAAGTCGTCAA	TACTCCCCACGATCCTGATC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.034460	129308	129860	1198-1198	0.500000	59.7
ndhA_p4	ndhA	4	TTGTTTGAGGCAAGTCGTCA	TACTCCCCACGATCCTGATC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.034460	129308	129860	1199-1199	0.500000	59.7
ndhA_p5	ndhA	5	TGCCAATCAACAACGGGTAA	AACGAGTGGATGGGTAGGAA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.070355	129308	129860	1627-1627	0.500000	59.4
ndhH_p1	ndhH	1	TATTCGGGGCCAATACGTTG	GTCAACGGCTGCTAGCTTAT	20	20	50.0	50.0	58.0	58.1	4.4	17.2	0.102378	131784	132965	1597-1597	0.500000	59.2
ndhH_p2	ndhH	2	TATTCGGGGCCAATACGTTG	ATCCATATCAGGGAAGGGGG	20	20	50.0	55.0	58.0	57.9	4.4	0.0	0.109501	131784	132965	1807-1807	0.500000	59.1
ndhH_p3	ndhH	3	TATTCGGGGCCAATACGTTG	CCATATCAGGGAAGGGGGAT	20	20	50.0	55.0	58.0	57.9	4.4	0.0	0.109501	131784	132965	1805-1805	0.500000	59.1
ndhH_p4	ndhH	4	CCAACGGGAGCAATACTTGA	GTCAACGGCTGCTAGCTTAT	20	20	50.0	50.0	57.9	58.1	0.0	17.2	0.166625	131784	132965	1831-1831	0.500000	58.7
ndhH_p5	ndhH	5	TCCAACGGGAGCAATACTTG	GTCAACGGCTGCTAGCTTAT	20	20	50.0	50.0	57.9	58.1	0.0	17.2	0.166625	131784	132965	1832-1832	0.500000	58.7
rps15-ycf1_p1	rps15-ycf1	1	GCTCAATCGTTCAGGTCCAT	AACCCCTTCGACTGTCTGTA	20	20	50.0	50.0	58.0	58.0	0.0	6.2	0.035208	133361	133718	1493-1493	0.500000	59.7
rps15-ycf1_p2	rps15-ycf1	2	GCTCAATCGTTCAGGTCCAT	ACCCCTTCGACTGTCTGTAA	20	20	50.0	50.0	58.0	58.0	0.0	6.2	0.035208	133361	133718	1492-1492	0.500000	59.7
rps15-ycf1_p3	rps15-ycf1	3	GCTCAATCGTTCAGGTCCAT	ATGGCGTGGATACCAATTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048582	133361	133718	1268-1268	0.500000	59.6
rps15-ycf1_p4	rps15-ycf1	4	GCTCAATCGTTCAGGTCCAT	ATTGGAATGGCGTGGATACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048582	133361	133718	1274-1274	0.500000	59.6
rps15-ycf1_p5	rps15-ycf1	5	GCTCAATCGTTCAGGTCCAT	GGAATGGCGTGGATACCAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048582	133361	133718	1271-1271	0.500000	59.6
ycf1_p1	ycf1	1	TGGGAAGAACCAAAGTTGCA	CCTTGTTTGCGATAAGGGGA	20	20	45.0	50.0	57.8	57.9	0.0	8.9	0.274286	134985	135104	389-389	0.500000	57.8
ycf1_p2	ycf1	2	TGGGAAGAACCAAAGTTGCA	TCCTTGTTTGCGATAAGGGG	20	20	45.0	50.0	57.8	57.9	0.0	14.4	0.274286	134985	135104	390-390	0.500000	57.8
ycf1_p3	ycf1	3	TGGGAAGAACCAAAGTTGCA	AAAATATCGCAAACCGGGGT	20	20	45.0	45.0	57.8	58.2	0.0	6.3	0.309451	134985	135104	906-906	0.500000	57.5
ycf1_p4	ycf1	4	TGGGAAGAACCAAAGTTGCA	AAATATCGCAAACCGGGGTT	20	20	45.0	45.0	57.8	58.2	0.0	6.3	0.309451	134985	135104	905-905	0.500000	57.5
ycf1_p5	ycf1	5	TGGGAAGAACCAAAGTTGCA	CTTCAGCGGTCTAGAACGAG	20	20	45.0	55.0	57.8	57.8	0.0	33.5	0.332928	134985	135104	762-762	0.500000	57.3
5S ribosomal RNA-4.5S ribosomal RNA_p1	5S ribosomal RNA-4.5S ribosomal RNA	1	AGTATCGTCACCGCAGTAGA	AGGCATCCTAACAGACCAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.075816	141113	141325	324-329	1.000000	83.1
5S ribosomal RNA-4.5S ribosomal RNA_p2	5S ribosomal RNA-4.5S ribosomal RNA	2	TATCGTCACCGCAGTAGAGT	AGGCATCCTAACAGACCAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.075816	141113	141325	322-327	1.000000	83.1
5S ribosomal RNA-4.5S ribosomal RNA_p3	5S ribosomal RNA-4.5S ribosomal RNA	3	TATCGTCACCGCAGTAGAGT	TGAGGCATCCTAACAGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.082895	141113	141325	324-329	1.000000	83.0
5S ribosomal RNA-4.5S ribosomal RNA_p4	5S ribosomal RNA-4.5S ribosomal RNA	4	AGTATCGTCACCGCAGTAGA	GTCAAGTGGAAGTGCAGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	141113	141325	356-361	1.000000	82.7
5S ribosomal RNA-4.5S ribosomal RNA_p5	5S ribosomal RNA-4.5S ribosomal RNA	5	TATCGTCACCGCAGTAGAGT	GTCAAGTGGAAGTGCAGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	141113	141325	354-359	1.000000	82.7