Hotspot

ErcaC_r001-trnI-GAU

Genome · 95312-95616 · 305 bp

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Pi 0.041

MarkerSeek score 73.10

Primer available yes

Label ErcaC_r001-trnI-GAU

Feature Metadata

label_name: ErcaC_r001-trnI-GAU
feature_type: igs
region: Genome
length_bp: 305
variable_sites: 22
pi: 0.041
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ErcaC_r001-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	388-395 bp	1.0	82.0
ErcaC_r001-trnI-GAU_p2	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	420-427 bp	1.0	81.8
ErcaC_r001-trnI-GAU_p3	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	55.0	50.0	57.7	57.8	439-446 bp	1.0	81.5
ErcaC_r001-trnI-GAU_p4	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	50.0	55.0	58.3	57.8	442-449 bp	1.0	81.4
ErcaC_r001-trnI-GAU_p5	AGTGACTGGAGTGAAGTCGT	CGCTGATGAGCACATTGAAC	50.0	50.0	58.3	57.8	461-468 bp	1.0	81.2