Hotspot

trnE-UUC-trnG-UCC

Genome · 30197-31234 · 1038 bp

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Pi 0.266

MarkerSeek score 76.80

Primer available yes

Label trnE-UUC-trnG-UCC

Feature Metadata

label_name: trnE-UUC-trnG-UCC
feature_type: igs
region: Genome
length_bp: 1038
variable_sites: 408
pi: 0.266
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnG-UCC_p1	TGGAGATTCCAATCGCTGTG	GCCTTCCCAAAATGAAAGGC	50.0	50.0	58.0	57.9	1840-2031 bp	0.6	75.0
trnE-UUC-trnG-UCC_p2	GGAGATTCCAATCGCTGTGT	GCCTTCCCAAAATGAAAGGC	50.0	50.0	58.0	57.9	1839-2030 bp	0.6	75.0
trnE-UUC-trnG-UCC_p3	TGGAGATTCCAATCGCTGTG	GCCTTTCATTTTGGGAAGGC	50.0	50.0	58.0	57.9	1645 bp	0.2	47.0
trnE-UUC-trnG-UCC_p4	GGAGATTCCAATCGCTGTGT	GCCTTTCATTTTGGGAAGGC	50.0	50.0	58.0	57.9	1644 bp	0.2	47.0
trnE-UUC-trnG-UCC_p5	TGGAGATTCCAATCGCTGTG	TTGCCCCCAAAATGAAAGGA	50.0	45.0	58.0	57.9	1688 bp	0.2	46.9