Hotspot

trnD-GUC-trnY-GUA

Genome · 29638-29980 · 343 bp

Back to Erodium

Pi 0.0446

MarkerSeek score 77.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: Genome
length_bp: 343
variable_sites: 27
pi: 0.0446
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	CTGGTTCAAATCCAGCTCGA	50.0	50.0	58.5	57.9	397-412 bp	1.0	82.8
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	TGGTTCAAATCCAGCTCGAC	50.0	50.0	58.5	58.2	396-411 bp	1.0	82.0
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	413-428 bp	1.0	81.4
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	412-427 bp	1.0	81.4
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	434-449 bp	1.0	80.1