Hotspot

ycf1

SSC · 125666-131146 · 5481 bp

Back to Eriostemon

Pi 0.0123

MarkerSeek score 45.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 67
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAATCCTCGGACCATTCGG	GAGAGGAGGCAGGGTCTTTA	50.0	55.0	58.0	58.1	794 bp	1.0	80.6
ycf1_p2	TGATTTGAGTTAGCGAGCCC	GAGAGGAGGCAGGGTCTTTA	50.0	55.0	58.0	58.1	1389 bp	1.0	80.3
ycf1_p3	TGATTTGAGTTAGCGAGCCC	CCCCGGTGGTCATACAAATT	50.0	50.0	58.0	57.9	1231 bp	1.0	80.3
ycf1_p4	TTTTCGGGGAGAACCAAAGG	GAGAGGAGGCAGGGTCTTTA	50.0	55.0	58.0	58.1	1052 bp	0.5	58.9
ycf1_p5	TTTTCGGGGAGAACCAAAGG	CCCCGGTGGTCATACAAATT	50.0	50.0	58.0	57.9	894 bp	0.5	58.8