Hotspot

ndhA

SSC · 121516-123753 · 2238 bp

Back to Eriostemon

Pi 0.00806

MarkerSeek score 57.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 18
pi: 0.00806
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCATAGGGAACATATCGTGA	ATGGGGGAAGTTGATCGTTG	42.9	50.0	55.9	57.9	2348-2351 bp	1.0	55.3
ndhA_p2	ACCATAGGGAACATATCGTGA	GGGGGAAGTTGATCGTTGAA	42.9	50.0	55.9	57.8	2346-2349 bp	1.0	54.8
ndhA_p3	ACCATAGGGAACATATCGTGAA	ATGGGGGAAGTTGATCGTTG	40.9	50.0	56.7	57.9	2348-2351 bp	1.0	53.3
ndhA_p4	ACCATAGGGAACATATCGTGAA	GGGGGAAGTTGATCGTTGAA	40.9	50.0	56.7	57.8	2346-2349 bp	1.0	52.8
ndhA_p5	ACCATAGGGAACATATCGTGA	GCCCACCGGGTTTTATCAAT	42.9	50.0	55.9	58.5	2446-2449 bp	1.0	52.1