Hotspot

clpP1

LSC · 71644-73732 · 2089 bp

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Pi 0.0106

MarkerSeek score 49.10

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2089
variable_sites: 22
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	CCCTTGTACATGTTCCTCGG	TCAAACGACCGGGATCATTC	55.0	50.0	58.0	58.0	2793-2800 bp	1.0	80.9
clpP1_p2	ACCCTTAAGGAGTCACTGCT	TCAAACGACCGGGATCATTC	50.0	50.0	58.0	58.0	2598-2605 bp	1.0	80.9
clpP1_p3	CCCTTGTACATGTTCCTCGG	CGCCCTCCCTTCTTTTCTTT	55.0	50.0	58.0	58.1	2665-2672 bp	1.0	80.4
clpP1_p4	ACCCTTAAGGAGTCACTGCT	CGCCCTCCCTTCTTTTCTTT	50.0	50.0	58.0	58.1	2470-2477 bp	1.0	80.4
clpP1_p5	ACCCTTAAGGAGTCACTGCT	CGGCTACACCTTCGTAACTC	50.0	55.0	58.0	58.1	2832-2839 bp	1.0	80.3