Hotspot

trnN-GUU-trnR-ACG

IRa · 131554-132165 · 612 bp

Back to Eriostemon

Pi 0.00656

MarkerSeek score 62.80

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 612
variable_sites: 4
pi: 0.00656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	GCTGTTAACTGACTGGTCGT	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.8	58.1	766-768 bp	1.0	78.3
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.2	58.1	753-755 bp	1.0	78.2
trnN-GUU-trnR-ACG_p3	TAGAATCTCCCGCTGTTCCT	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.8	58.1	811-813 bp	1.0	78.2
trnN-GUU-trnR-ACG_p4	GGTTGAGAGCGGGAATTGAA	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.2	58.1	841-843 bp	1.0	78.0
trnN-GUU-trnR-ACG_p5	CTGGTCGTAGGTTCGAATCC	TAGGGTACTTCCGGGAACAG	55.0	55.0	57.8	58.1	754-756 bp	1.0	77.8