Hotspot

trnR-ACG-trnN-GUU

IRb · 110110-110721 · 612 bp

Back to Eriostemon

Pi 0.00656

MarkerSeek score 62.80

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 612
variable_sites: 4
pi: 0.00656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	TAGGGTACTTCCGGGAACAG	GCTGTTAACTGACTGGTCGT	55.0	50.0	58.1	57.8	766-768 bp	1.0	78.3
trnR-ACG-trnN-GUU_p2	TAGGGTACTTCCGGGAACAG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.1	58.2	753-755 bp	1.0	78.2
trnR-ACG-trnN-GUU_p3	TAGGGTACTTCCGGGAACAG	TAGAATCTCCCGCTGTTCCT	55.0	50.0	58.1	57.8	811-813 bp	1.0	78.2
trnR-ACG-trnN-GUU_p4	TAGGGTACTTCCGGGAACAG	GGTTGAGAGCGGGAATTGAA	55.0	50.0	58.1	58.2	841-843 bp	1.0	78.0
trnR-ACG-trnN-GUU_p5	TAGGGTACTTCCGGGAACAG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	58.1	57.8	754-756 bp	1.0	77.8