Hotspot

psbC-trnS-UGA

LSC · 36805-37053 · 249 bp

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Pi 0.0363

MarkerSeek score 73.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 249
variable_sites: 9
pi: 0.0363
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCGATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.3	58.1	355-356 bp	1.0	79.7
psbC-trnS-UGA_p2	CCTGTTCTTTCGATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.3	58.2	301-302 bp	1.0	78.8
psbC-trnS-UGA_p3	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	432-433 bp	1.0	78.0
psbC-trnS-UGA_p4	ACCTGTTCTTTCGATGACCC	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.5	58.1	356-357 bp	1.0	78.0
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.4	58.2	378-379 bp	1.0	77.0