Hotspot

trnC-GCA-petN

LSC · 28651-29377 · 727 bp

Back to Eriostemon

Pi 0.0124

MarkerSeek score 65.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 9
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.4	58.1	849-850 bp	1.0	77.1
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	CCATACTACGAGCGAAAGGG	50.0	55.0	58.4	57.9	856-857 bp	1.0	76.9
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	CCCATACTACGAGCGAAAGG	50.0	55.0	58.4	57.9	857-858 bp	1.0	76.9
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CCCCATACTACGAGCGAAAG	50.0	55.0	58.4	57.9	858-859 bp	1.0	76.9
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	811-812 bp	1.0	76.8