Hotspot

atpH-atpI

LSC · 13913-15047 · 1135 bp

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Pi 0.0125

MarkerSeek score 52.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1135
variable_sites: 14
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	AGCCTTGCTCACGTCAATAG	45.0	50.0	58.0	58.0	1492-1501 bp	1.0	81.0
atpH-atpI_p2	GGAGATTCCTGCCAAGAAGG	AGCCTTGCTCACGTCAATAG	55.0	50.0	58.0	58.0	2004-2019 bp	1.0	80.7
atpH-atpI_p3	CCCTCGTTTGTTGCAACATC	AGCCTTGCTCACGTCAATAG	50.0	50.0	57.9	58.0	1939-1954 bp	1.0	80.7
atpH-atpI_p4	CCTCGTTTGTTGCAACATCC	AGCCTTGCTCACGTCAATAG	50.0	50.0	57.9	58.0	1938-1953 bp	1.0	80.7
atpH-atpI_p5	CTCGTTTGTTGCAACATCCC	AGCCTTGCTCACGTCAATAG	50.0	50.0	57.9	58.0	1937-1952 bp	1.0	80.7