Hotspot

atpF-atpH

LSC · 13180-13666 · 487 bp

Back to Eriostemon

Pi 0.021

MarkerSeek score 56.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 487
variable_sites: 10
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGGTCCAGCCTATTCTTGA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	900-902 bp	1.0	81.5
atpF-atpH_p2	GGTGCTTTGTCTGGACTTCA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	1116-1119 bp	1.0	81.1
atpF-atpH_p3	TGGGTCCAGCCTATTCTTGA	TAGTTACTTCGGCTGGGTGA	50.0	50.0	58.0	58.1	1028-1030 bp	1.0	81.1
atpF-atpH_p4	GGTGCTTTGTCTGGACTTCA	TAGTTACTTCGGCTGGGTGA	50.0	50.0	58.0	58.1	1244-1247 bp	1.0	80.8
atpF-atpH_p5	GGTGCTTTGTCTGGACTTCA	GTTACTTCGGCTGGGTGAAT	50.0	50.0	58.0	57.9	1242-1245 bp	1.0	80.5