Hotspot

trnH-GUG-psbA

LSC · 125-509 · 385 bp

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Pi 0.0674

MarkerSeek score 78.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 23
pi: 0.0674
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ACTTCCCTCTAGACCTAGCT	55.0	50.0	58.1	56.2	432-457 bp	1.0	72.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	ACTTCCCTCTAGACCTAGCT	50.0	50.0	57.9	56.2	446-471 bp	1.0	72.2
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	ACTTCCCTCTAGACCTAGCT	50.0	50.0	57.9	56.2	445-470 bp	1.0	72.2
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	ACTTCCCTCTAGACCTAGCT	50.0	50.0	57.9	56.2	447-472 bp	1.0	72.2
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	AACTTCCCTCTAGACCTAGCT	55.0	47.6	58.1	56.9	433-458 bp	1.0	70.7