Hotspot

ycf4

LSC · 59587-60165 · 579 bp

Back to Eriosema

Pi 0.00173

MarkerSeek score 59.30

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 579
variable_sites: 1
pi: 0.00173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	AGTGCCAAGTTACTTTAGAAACA	ACAATTCTGAAGCTTTTTGTGCA	34.8	34.8	56.3	58.2	725 bp	1.0	40.1
ycf4_p2	AGTGCCAAGTTACTTTAGAAACA	GACAATTCTGAAGCTTTTTGTGC	34.8	39.1	56.3	57.7	726 bp	1.0	40.1
ycf4_p3	AGTGCCAAGTTACTTTAGAAACA	CAATTCTGAAGCTTTTTGTGCA	34.8	36.4	56.3	56.6	724 bp	1.0	40.1
ycf4_p4	AGTGCCAAGTTACTTTAGAAACA	ACAATTCTGAAGCTTTTTGTGC	34.8	36.4	56.3	56.6	725 bp	1.0	40.1
ycf4_p5	AGTGCCAAGTTACTTTAGAAACA	TTCTGAAGCTTTTTGTGCAA	34.8	35.0	56.3	54.5	721 bp	1.0	40.1