Hotspot

ycf1

SSC · 122814-128189 · 5376 bp

Back to Eriosema

Pi 0.00167
MarkerSeek score 53.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5376
variable_sites
9
pi
0.00167
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TGCAATAGAAAAGAAAAAGAGAGGT GGAGGTGGGCGAATTTCTTC 32.0 55.0 57.2 58.9 591-615 bp 1.0 43.2
ycf1_p2 TGCAATAGAAAAGAAAAAGAGAGGT GGAGGTGGGCGAATTTCTT 32.0 52.6 57.2 57.7 591-615 bp 1.0 43.2
ycf1_p3 TGCAATAGAAAAGAAAAAGAGAGGTT GGAGGTGGGCGAATTTCTTC 30.8 55.0 57.8 58.9 591-615 bp 1.0 43.2
ycf1_p4 TTGCAATAGAAAAGAAAAAGAGAGGT GGAGGTGGGCGAATTTCTTC 30.8 55.0 57.8 58.9 592-616 bp 1.0 43.2
ycf1_p5 TGCAATAGAAAAGAAAAAGAGAGG GGAGGTGGGCGAATTTCTTC 33.3 55.0 55.6 58.9 591-615 bp 1.0 43.2
ycf1_p1 TGGAATGGTTGCTTGGGAAA AGTTCGAAAAATTTTAAGGGAA 45.0 27.3 57.9 52.5 226 bp 1.0 40.7
ycf1_p2 TTGGAATGGTTGCTTGGGAA AGTTCGAAAAATTTTAAGGGAA 45.0 27.3 57.9 52.5 227 bp 1.0 40.7
ycf1_p3 TGGAATGGTTGCTTGGGAAA TCTAGTTCGAAAAATTTTAAGGGA 45.0 29.2 57.9 54.5 229 bp 1.0 40.7
ycf1_p4 TTGGAATGGTTGCTTGGGAA TCTAGTTCGAAAAATTTTAAGGGA 45.0 29.2 57.9 54.5 230 bp 1.0 40.7
ycf1_p5 TGGAATGGTTGCTTGGGAAA AGTTCGAAAAATTTTAAGGGAAA 45.0 26.1 57.9 53.3 226 bp 1.0 40.7