Hotspot

petD

LSC · 76272-77485 · 1214 bp

Back to Eriosema

Pi 0.000824

MarkerSeek score 59.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1214
variable_sites: 1
pi: 0.000824
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGGGAACTCTCCGA	ACACAATGGACATGACATGA	50.0	40.0	58.0	55.0	1292 bp	1.0	55.6
petD_p2	TGAAGGGAACTCTCCGAAGA	ACACAATGGACATGACATGA	50.0	40.0	57.7	55.0	1289 bp	1.0	53.2
petD_p3	AGTTGAAGGGAACTCTCCGA	ACACAATGGACATGACATGAT	50.0	38.1	58.0	55.6	1292 bp	1.0	52.4
petD_p4	AGTTGAAGGGAACTCTCCGA	TCCCTAGATACACAATGGACA	50.0	42.9	58.0	55.5	1301 bp	1.0	51.8
petD_p5	AGTTGAAGGGAACTCTCCGA	CCCTAGATACACAATGGACATGA	50.0	43.5	58.0	57.4	1300 bp	1.0	51.6