Hotspot

petB

LSC · 74580-76071 · 1492 bp

Back to Eriosema

Pi 0.00201

MarkerSeek score 59.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1492
variable_sites: 3
pi: 0.00201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGATTTGGTAGTTTGATCGTGG	TGAAATTGATTCCTCCCCAA	45.5	40.0	57.4	54.5	1606 bp	1.0	40.1
petB_p2	GGATTTGGTAGTTTGATCGTGG	TGAAATTGATTCCTCCCCAAA	45.5	38.1	57.4	55.3	1606 bp	1.0	40.1
petB_p3	GGATTTGGTAGTTTGATCGTGG	ATGAAATTGATTCCTCCCCA	45.5	40.0	57.4	54.2	1607 bp	1.0	40.1
petB_p4	GGATTTGGTAGTTTGATCGTGG	TGAAATTGATTCCTCCCCAAAA	45.5	36.4	57.4	56.1	1606 bp	1.0	40.1
petB_p5	GGATTTGGTAGTTTGATCGTGG	ATGAAATTGATTCCTCCCCAA	45.5	38.1	57.4	55.1	1607 bp	1.0	40.1