Hotspot

matK

LSC · 2206-3723 · 1518 bp

Back to Eriosema

Pi 0.000659

MarkerSeek score 59.10

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 1
pi: 0.000659
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACCTATTTACACCGATTTACAAACT	CCTTGTTTTGACTGTATCGCA	32.0	42.9	56.8	56.8	1666 bp	1.0	40.0
matK_p2	ACCTATTTACACCGATTTACAAACT	CCTTGTTTTGACTGTATCGCAC	32.0	45.5	56.8	58.2	1666 bp	1.0	40.0
matK_p3	ACACCGATTTACAAACTATTCTAACC	CCTTGTTTTGACTGTATCGCA	34.6	42.9	57.7	56.8	1658 bp	1.0	40.0
matK_p4	ACACCGATTTACAAACTATTCTAACC	CCTTGTTTTGACTGTATCGCAC	34.6	45.5	57.7	58.2	1658 bp	1.0	40.0
matK_p5	ACCTATTTACACCGATTTACAAACT	TCCTTGTTTTGACTGTATCGCA	32.0	40.9	56.8	58.3	1667 bp	1.0	40.0